H2N2V1, Main, Exploration, bibRecord, 002E21

[Growth characteristics of single gene mutants of A/Leningrad/134/57(H2N2) influenza virus and cold-adapted mutant A/Leningrad/134/17/57(H2N2)].

Identifieur interne : 002E21 ( Main/Exploration ); précédent : 002E20; suivant : 002E22

[Growth characteristics of single gene mutants of A/Leningrad/134/57(H2N2) influenza virus and cold-adapted mutant A/Leningrad/134/17/57(H2N2)].

Auteurs : A R Rekstin ; I V Kiseleva ; Iu A. Desheva ; D. Kats ; G I Aleksandrova ; A I Klimov ; L G Rudenko

Source :

Voprosy virusologii [ 0507-4088 ]

RBID : pubmed:17500232

Descripteurs français

KwdFr :
- Adaptation physiologique, Animaux, Basse température, Embryon de poulet, Gènes viraux (physiologie), Lignée cellulaire, Mutation ponctuelle, Protéines de la matrice virale (génétique), Protéines virales (génétique), Protéines virales non structurales (génétique), RNA replicase (génétique), Sous-type H2N2 du virus de la grippe A (croissance et développement), Sous-type H2N2 du virus de la grippe A (génétique).
MESH :
- croissance et développement : Sous-type H2N2 du virus de la grippe A.
- génétique : Protéines de la matrice virale, Protéines virales, Protéines virales non structurales, RNA replicase, Sous-type H2N2 du virus de la grippe A.
- physiologie : Gènes viraux.
- Adaptation physiologique, Animaux, Basse température, Embryon de poulet, Lignée cellulaire, Mutation ponctuelle.

English descriptors

KwdEn :
- Adaptation, Physiological, Animals, Cell Line, Chick Embryo, Cold Temperature, Genes, Viral (physiology), Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (growth & development), Point Mutation, RNA Replicase (genetics), Viral Matrix Proteins (genetics), Viral Nonstructural Proteins (genetics), Viral Proteins (genetics).
MESH :
- chemical , genetics : RNA Replicase, Viral Matrix Proteins, Viral Nonstructural Proteins, Viral Proteins.
- genetics : Influenza A Virus, H2N2 Subtype.
- growth & development : Influenza A Virus, H2N2 Subtype.
- physiology : Genes, Viral.
- Adaptation, Physiological, Animals, Cell Line, Chick Embryo, Cold Temperature, Point Mutation.

Abstract

The authors examined a role of some mutated A/Leningrad/134/17/57(H2N2) virus genes in the realization of growth characteristics. The latter of single gene reassortants (SGRs) (PB2, PB1, PA, M, and NS), epidemic virus and attenuation donor were assessed by infecting MDCK cells and hen embryos at a low inoculation index. Viral replication in the hen embryos and cultured tissue was compared at 34 degrees C. The viruses and reassortants tested showed a high growth capacity in the hen embryos (9.5-10.5 Ig TCID50). The growth curves of viruses were studied on the cultured MDCK cells at a low inoculation index indicated that Len/17 and the single gene reassortants M and NS had the highest growth capacity. At the same time the growth of both PB1 and PB2 SGRs was less extensive. The reproduction of PB2 SGR was 100-1000 times less than that of other viruses tested. M, NS, and PA gene mutations did not affect viral growth in hen embryos and cultured tissue while PB2 gene mutation and its constellations with other genes caused a reduction in viral growth in the cultured tissue.

PubMed: 17500232

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">The authors examined a role of some mutated A/Leningrad/134/17/57(H2N2) virus genes in the realization of growth characteristics. The latter of single gene reassortants (SGRs) (PB2, PB1, PA, M, and NS), epidemic virus and attenuation donor were assessed by infecting MDCK cells and hen embryos at a low inoculation index. Viral replication in the hen embryos and cultured tissue was compared at 34 degrees C. The viruses and reassortants tested showed a high growth capacity in the hen embryos (9.5-10.5 Ig TCID50). The growth curves of viruses were studied on the cultured MDCK cells at a low inoculation index indicated that Len/17 and the single gene reassortants M and NS had the highest growth capacity. At the same time the growth of both PB1 and PB2 SGRs was less extensive. The reproduction of PB2 SGR was 100-1000 times less than that of other viruses tested. M, NS, and PA gene mutations did not affect viral growth in hen embryos and cultured tissue while PB2 gene mutation and its constellations with other genes caused a reduction in viral growth in the cultured tissue.</div>
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<name sortKey="Kats, D" sort="Kats, D" uniqKey="Kats D" first="D" last="Kats">D. Kats</name>
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<name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name>
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[Growth characteristics of single gene mutants of A/Leningrad/134/57(H2N2) influenza virus and cold-adapted mutant A/Leningrad/134/17/57(H2N2)].

[Growth characteristics of single gene mutants of A/Leningrad/134/57(H2N2) influenza virus and cold-adapted mutant A/Leningrad/134/17/57(H2N2)].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
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